Regenerative medicine using cells is highly reliant on the ability to grow and expand cells outside the body (ex vivo). Researchers are constantly trying to improve the conditions by which cells are grown, expanded and stored to in an effort to optimize their utility in cell based therapies. ADH-01’s composition has been shown to enhance cell viability, growth, and expansion. As such, the properties of ADH-01 could lead to improved applications for basic research through regenerative medical applications for patients.
Glycosaminoglycans (GAGs) are key components of the extracellular matrix (ECM), which include hyaluronic acid and chondroitin sulfate. These GAGs form an important component connective tissues, and have an important role in the regulation of cell physiology and pathophysiology. GAGs have physical properties, which can provide structural integrity to cells and allow for cell migration between cells in the tissues. These physical properties are important when considering cell viability, expansion and growth in vivo and ex vivo. In addition, glucosamine has been shown to have a role in the chondrocyte production of important factors including aggrecan, collagen type II, and the components of the cartilage matrix. ADH-01 is comprised of hyaluronic acid, chondroitin sulfate, and n-acetyl-d-gucosamine, which proposes to have superior properties in the maintaining cell viability, and the growth and expansion of various cell lines used in regenerative medicine.